Journal: PLoS ONE
Article Title: C5a Receptor Deficiency Alters Energy Utilization and Fat Storage
doi: 10.1371/journal.pone.0062531
Figure Lengend Snippet: Gene expression for immune factors CD11c (A) and F4/80 (B) and for lipid-related factors fatty acid transporter ( CD36 ), fatty acid synthase ( FAS ), lipoprotein lipase ( LPL ), diacylglycerolacyltransferase-1 ( DGAT1 ), diacylglycerolacyltransferase-2 ( DGAT2 ) and peroxisome-proliferator activated receptor gamma ( PPARG ) (G) were evaluated in WT CHOW diet (open bars), C5aRKO CHOW (solid bars), WT DIO diet (checkered bars) and C5aRKO DIO (striped bars) in gonadal adipose tissue, where n = 6–12 per group. MCP-1 (C) and KC (D) secretion were measured in ex vivo adipose tissue in WT (white bars) and C5aRKO (diagonally striped bars) mice following overnight (24h) treatment with C5a (50 ng/mL), LPS, or both vs control (CTL) where n = 8–10 per group. Ex vivo C3 secretion (E) and ASP production (F) were measured in media from adipose tissue of WT CHOW diet, C5aRKO CHOW, WT DIO and C5aRKO DIO incubated for 24 h. Results are expressed as means ± SEM; statistical differences were determined by ANOVA followed by Bonferroni post-test, where *P<0.05, **P<0.001 and ***P<0.0001 vs CTL (C, D) or vs WT on their respective diet (A,B,E-G) unless otherwise indicated.
Article Snippet: Plasma C5a was measured using a sandwich ELISA (DuoSet ELISA, cat DY2150) from R&D Systems (Minneapolis, MN, USA).
Techniques: Gene Expression, Ex Vivo, Control, Incubation